Numero 162

Cari socie e soci,

siamo pronti ad iniziare i nostri VIRTUAL CLUB SIBV, una serie di webinar dedicati a giovani studenti, dottorandi e ricercatori.

Il primo seminario si svolgerà il 19 maggio 2022 dalle ore 17:00 alle ore 18:00 e sarà svolto dal Dr. Matteo Grenzi dell’Università degli Studi di Milano.

Long-distance turgor changes induce systemic activation of plant GLUTAMATE RECEPTOR-LIKE channels

Matteo Grenzi, Ambra Selene Parmagnani, Stefano Buratti, Ilaria Abdel Aziz, Iwona BernackaWojcik, Francesca Resentini, Jan Šimura, Fabrizio Gandolfo Doccula, Andrea Alfieri, Laura Luoni, Karin Ljung, Maria Cristina Bonza, Eleni Stavrinidou, Alex Costa 

Permanently restricted to their site of germination, plants evolved mechanisms to react to ever-changing environmental conditions and harmful stresses. Local damage triggers inducible defence mechanisms that are often induced systemically in organs that are not damaged yet. Systemic responses are mediated by long-distance signalling that requires the activity of Glutamate Receptor-Like channels (GLRs). GLRs are homologs of animal Ionotropic Glutamate Receptors (iGluRs) which are ligand-gated cation channels in the central nervous system. Even though iGluRs are gated through the binding with the L-Glutamate, the mechanism throughout GLRs is activated in planta is poorly understood. As an example, we still do not know if the GLRs binding of amino acids is necessary for their activity. We took the advantage of the recently obtained crystal structure of the Arabidopsis thaliana AtGLR3.3 Ligand Binding Domain (LBD) to identify residues involved in the amino acid-binding. We, therefore, introduced single point mutations in the genome sequence of the AtGLR3.3 gene to prevent or abolish its amino acid-binding, and with the obtained constructs we complemented the glr3.3 KO. By combining high-end imaging, genetics, and bioelectronics we prove that leaf injury, such as wound and burn, and root-applied hypoosmotic stress induce the systemic apoplastic increase of L-Glutamate that activates GLR channels through their LBD. In addition, our work supports the evidence that long-distance signalling is governed by a systemic change in the turgor state and that GLRs are downstream of it.

Puoi partecipare all’evento telematico utilizzando questo link (ndr link presente su NL inviata)

Ciascuna sessione dei Virtual Club sarà svolta attraverso l’uso della Piattaforma Microsoft Teams.

Informazioni relative alle prossime sessioni sono presenti sul sito

http://www.sibv.eu/congressi-ed-eventi/virtual-club-sibv

Per ulteriori informazioni non esitate a contattarci inviandoci una e-mail all’indirizzo segretario@sibv.eu

Ci auguriamo ed auspichiamo una grande partecipazione tra i soci ed interazione con gli speaker al fine di rendere questi momenti di incontro virtuale una preziosa occasione per la crescita della nostra comunità,

Un caro saluto

 

Dear SIBV members and associates,

we are ready to start our VIRTUAL CLUB SIBV, a series of webinars dedicated to young students, PhD students and researchers.

The first seminar will take place on May 19, 2022 from 17:00 to 18:00 and will be held by Dr. Matteo Grenzi of the University of Milan.

Long-distance turgor changes induce systemic activation of plant GLUTAMATE RECEPTOR-LIKE channels

Matteo Grenzi, Ambra Selene Parmagnani, Stefano Buratti, Ilaria Abdel Aziz, Iwona BernackaWojcik, Francesca Resentini, Jan Šimura, Fabrizio Gandolfo Doccula, Andrea Alfieri, Laura Luoni, Karin Ljung, Maria Cristina Bonza, Eleni Stavrinidou, Alex Costa 

Permanently restricted to their site of germination, plants evolved mechanisms to react to ever-changing environmental conditions and harmful stresses. Local damage triggers inducible defence mechanisms that are often induced systemically in organs that are not damaged yet. Systemic responses are mediated by long-distance signalling that requires the activity of Glutamate Receptor-Like channels (GLRs). GLRs are homologs of animal Ionotropic Glutamate Receptors (iGluRs) which are ligand-gated cation channels in the central nervous system. Even though iGluRs are gated through the binding with the L-Glutamate, the mechanism throughout GLRs is activated in planta is poorly understood. As an example, we still do not know if the GLRs binding of amino acids is necessary for their activity. We took the advantage of the recently obtained crystal structure of the Arabidopsis thaliana AtGLR3.3 Ligand Binding Domain (LBD) to identify residues involved in the amino acid-binding. We, therefore, introduced single point mutations in the genome sequence of the AtGLR3.3 gene to prevent or abolish its amino acid-binding, and with the obtained constructs we complemented the glr3.3 KO. By combining high-end imaging, genetics, and bioelectronics we prove that leaf injury, such as wound and burn, and root-applied hypoosmotic stress induce the systemic apoplastic increase of L-Glutamate that activates GLR channels through their LBD. In addition, our work supports the evidence that long-distance signalling is governed by a systemic change in the turgor state and that GLRs are downstream of it. 

You can participate in the online event using this link (ndr link sent by email)

Each Virtual Club session will be held via Microsoft Teams.

Information on upcoming sessions can be found on the SIBV website 

http://www.sibv.eu/congressi-ed-eventi/virtual-club-sibv

For more information, do not hesitate to contact us by sending an e-mail to segretario@sibv.eu

We hope for great participation among the members and interaction with the speakers in order to make these moments of virtual meeting a precious opportunity for the growth of our community,

Warm greetings

 

Il Presidente, Laura De Gara                                                      Il Segretario, Sara Cimini